Heterologous expression of biologically active Mambalgin-1 peptide as a new potential anticancer, using a PVX-based viral vector in Nicotiana benthamiana
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چکیده
منابع مشابه
Transient expression of the cancer/testis cancer antigen NY-ESO-1 in Nicotiana benthamiana using a PVX-based viral vector
Background Cancer/testis antigens is a group of proteins which expression is usually restricted to the human germ line but is also present at high levels in several types of cancer cells [1]. The antigen NY-ESO-1 is one of these antigens showing high immunogenicity and, therefore, holding great potential for a cancer vaccine [2]. Its heterologous expression is being tested in different expressi...
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Background: The potential of plants, as a safe and eukaryotic system, is considered in the production of recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (PTGS) response in this new technology. The use of viral suppressors of gene silencing can prevent PTGS and improve transient expression level ...
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RNA viral vectors for improved Agrobacterium-mediated transient expression of heterologous proteins in Nicotiana benthamiana cell suspensions and hairy roots
BACKGROUND Plant cell suspensions and hairy root cultures represent scalable protein expression platforms. Low protein product titers have thus far limited the application of transient protein expression in these hosts. The objective of this work was to overcome this limitation by harnessing A. tumefaciens to deliver replicating and non-replicating RNA viral vectors in plant tissue co-cultures....
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ژورنال
عنوان ژورنال: Plant Cell, Tissue and Organ Culture (PCTOC)
سال: 2020
ISSN: 0167-6857,1573-5044
DOI: 10.1007/s11240-020-01838-x